BRIG is capable of using raw sequencing reads as query sequences to provide rapid preliminary insights into unassembled draft genome or meta-genome data. To illustrate this feature, we have simulated unassembled Illumina data using MetaSim [21] by randomly sampling one million 100 base pair sequences from the complete genome sequences shown in Figure 3A and applying an Illumina error model. Reads were translated into peptides and used as query sequences in BRIG (using BLASTx) to search against the same central reference sequence in Figure 3A, producing the image shown in Figure 3B. Despite being based only on raw sequencing reads, the representation of sequence presence, absence and variation in Figure 3B is highly similar to that found when using whole genome sequences in Figure 3A.
Trixie Model Set 219 Download
2ff7e9595c
Comments